Article Sidebar

Submitted
Nov 16, 2021
Published
Feb 15, 2022
Download
Pdf
Statistic
Read Counter : 813 Download : 1396

Downloads

Download data is not yet available.

Main Article Content

Abstract

Breast cancer is increasing as the constraints of side effects, drug-resistant, and high medical costs. Indonesia has a variety of marine biotas that can be explored. The sponge is one of the marines biotas that had potential candidates for anticancer compounds. The secondary metabolites of sponge such as steroids, alkaloids, polyketides, terpenoids, and polyacetylenes have pharmacology effects as anti-inflammation, antineoplastic and cytotoxic activity. The study is aimed to determine the secondary metabolite of Petrosian sp. ethanol extract and the cytotoxic activity at the T47D cell line of breast cancer. The ethanol extract of Petrosian sp. is obtained by maceration using 96% ethanol as a solvent. Extract weight is obtained 274.7 grams with a 2.94% yield of values. Cytotoxicity assay was using MTT methods with IC50 parameters by using software GraphPad prism version 5. Secondary metabolites of Petrosian sp. ethanol extract showing alkaloids, flavonoids, steroids, tannins, and saponins. The possible compound that showed anticancer activity is spinasterol. Cytotoxicity activities of Petrosian sp. ethanol extracts showing IC50 values at 78.13 ppm and IC50 values at 65.88 ppm on positive control. These results proved cytotoxicity activities had moderate cytotoxicity.


 

Keywords

Breast cancer T472 cell line Anticancer IC50 MTT method Petrosia sp

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

References

  1. Abdel-Lateff, A., Alarif, W.M., Al-Lihaibi, S.S., Abdel-Naim, A.B. (2017). Antiproliferative Effects of Selected Marine Organisms Collected from Red Sea. Pak J Pharm Sci, 30(2), 381-386.
  2. Adjeng, A. N. T., Hairah, S., Herman, S., Ruslin, R., Fitrawan, L. O. M., Sartinah, A., Ali, N. F. M., Sabarudin, S. (2020). Skrining Fitokimia dan Evaluasi Sediaan Sabun Cair Ekstrak Etanol 96% Kulit Buah Salak Pondoh (Salacca zalacca (Gaertn.) Voss.) Sebagai Antioksidan. Pharmauho: Jurnal Farmasi, Sains, Dan Kesehatan, 5(2), 3-6.
  3. Ambarwati, Erlinda K W. (2014). Efek Samping Kemoterapi Secara Fisik Pasien Penderita Kanker Serviks. Jurnal dan Prosiding Nasional Unismuh. American Cancer Society. (2019). Breast Cancer Basic Facts & Figures 2019-2020. American Cancer Society.
  4. Apriyandri, R. A & Yuni E. (2019). Artikel Ulasan: Aktivitas Kandungan Senyawa Dan Karakteristik Spons Laut Genus Petrosia, Farmaka.17(2). 285-295.
  5. Bols A., Kawano L.E.J., Lee. (2011). December 2011. Cellular, Molecular, Genomics, And Biomedical Approaches Culture of Fish Cell Lines. Encyclopedia of Fish.
  6. Darmawan, Reina M., Budi R. (2019). Gambaran Hubungan Regimen Dosis dan Efek Samping Kemoterapi pada Pasien Kanker di RSUD Prof. Dr. Margono Soekarjo Purwokerto Periode Bulan Januari-Februari Tahun 2019. Majalah Farmaseutik, 15(20), 113-121.
  7. Dewi, D.A.D.Y, Astuti, K.W, Warditiani, N.K. (2013). Identifikasi Kandungan Kimia Ekstrak Kulit Buah Manggis (Garcinia mangostana L.). Jurnal OJS Unud
  8. Haedar, Baru S., Ratna D.P. (2016). Potensi Keanekaragaman Jenis Dan Sebaran Spons Di Perairan Pulau Saponda Laut Kabupaten Konawe. Sapa Laut,1(1), 1-9.
  9. Handayani, D., Mega, Y., Yohanes, A., dan Nicole J. de. Voogd. (2012). Isolasi Senyawa Sitotoksik Dari Spons Laut Petrosia sp. JPB Perikanan, 7(1), 69-76.
  10. Molaee, N., Ghasem, M., Abbas, P., Mostafa, E., Ali, G. (2017). Evaluating the Proliferation of Human Peripheral Blood Mononuclear Cells Using MTT Assay. International Journal of Basic Science In Medicine, 2(1), 25-28.
  11. Padmasari, P.D., Astuti, K.W., Warditiani, N.K. (2013). Skrining Fitokimia Ekstrak Etanol 70% Rimpang Bangle (Zingiber purpureum Roxb.). Jurnal Jurusan Farmasi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Udayana, 2(4), 1-7.
  12. Pailee, P., Mahidol, C., Ruchirawat, S., Prachyawarakorn, V. (2017). Sterols from Thai Marine Sponge Petrosia (Strongylophora) sp., and Their Cytotoxicity. Marine Drugs, 15(3), 54-61.
  13. Pertiwi, Dessy, A., Linosefa. (2020). Pengaruh Ekstrak Daun Sirsak (Annona muricata lin) Terhadap Viabilitas Cell Line Kanker Payudara T47D Secara In Vitro. Jurnal Kesehatan Andalas, 9(15), 165-168.
  14. Pratama, Rina, F. N. (2018). Review: Senyawa Aktif Antikanker Dari Bahan Alam Dan Aktivitasnya. Farmaka, 16(1), 30-36.
  15. Sagrero, A., Moisés, N., Eduardo, R.B., Carmen, L.Z., Manuel, J.E., Ramón, E.R.Z. (2017). Antiproliferative activity of spinasterol isolated of Stegnosperma halimifolium (Benth, 1844). Saudi Pharmaceutical Journal, 25(8), 1137-1143.
  16. Sajjadi, S. E., Mustafa, G., Mehrangiz, H., Leili, M. (2015). Cytotoxic effect of Cousinia verbascifolia Bunge against OVCAR-3 and HT-29 cancer cells. Journal of HerbMed Pharmacology, 4(1), 15-19.
  17. Sedky, N. K., El Gammal, Z. H., Wahba, A. E., Mosad, E., Waly, Z. Y., El-Fallal, A. A., Arafa, R. K., & El-Badri, N. (2018). The molecular basis of cytotoxicity of α-spinasterol from Ganoderma resinaceum: Induction of apoptosis and overexpression of p53 in breast and ovarian cancer cell lines. Journal of cellular biochemistry, 119(5), 3892–3902.
  18. Setiawan, Elvi, R.P.W., Ary, K. (2016). Sitotoksisitas Fraksi Nonpolar Brucea javanica (L.) Merr. Terhadap Sel Kanker Payudara T47D. Jurnal Penelitian Sains, 18(3), 111-115.
  19. Suzery, M., & Cahyono, B. (2014). Evaluation of Cytotoxicity Effect of Hyptis pectinata Poit (Lamiaceae) extracts using BSLT and MTT Methods. Jurnal Sains Dan Matematika, 22(3), 84-88.
  20. Wahyuni., Yusuf. M. I., Malik. F., Lubis. A. F., Indalifiany. A., Sahidin. I. (2019). Efek Imunomodulator Ekstrak Etanol Spons Melophlus sarasinorum Terhadap Aktivitas Fagositosis Sel Makrofag Pada Mencit Jantan Balb/C. Jurnal Farmasi Galenika: Galenika Journal of Pharmacy, 5(2), 147-157.
  21. World Health Organization. 2020. Cancer Country Profiles 2020. Retrieved April 6, 2021, from https://www.who.int/cancer/country-profiles/IDN_2020.pdf?ua=1.