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Sep 20, 2019
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Dec 20, 2019
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vol 5 no 2
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Abstract

White water lily (Nymphaeae alba) is one of the aquatic plants that is often used for traditional medicine. This plant has anticancer and antioxidant activity. The final result of this experiment is to determine the antibacterial activity of acetone and ethyl acetate extract from several parts of this plant, including leaves, stems and rhizomes againts  several types of bacterial including Staphylococcus aureus and Escherichia coli.  Kirby-Bauer diffusion Disc method is used in this study. Simplicia of leaves, stems and  rhizomes of white water lily were macerated with acetone and ethyl acetate for 3 x 24 hours. Antibacterial tests were carried out to acetone and ethyl acetate leaves, stems and rhizome extract of white water lily.  Amoxicillin was used  as a positive control and dimethyl sulfoxide (DMSO) with concentration 20% as a negative control. The extract and amoxicillin were diluted with 20% DMSO. Each disk contained 100 µg of extract and 10 µg of amoxicillin. Antibacterial tests that have been carried out show that the acetone extract of leaves, stems and rhizomes white water lily has the highest attack value against bacteria, the diameter of the inhibition zone is 20 mm against S. aureus Acetone and ethyl acetate leaves, stems and rhizomes extract of white water lily did not have inhibitory effect on E. coli.  

Keywords

White water lily, antibacterial, inhibition zone

Article Details

References

  1. Abirami, A., Nagarani, G., Saipriya, V., Ashwathi, R., & Siddhuraju, P. (2017). Influence of Thermal Treatments on Polyphenolic Contents and Antioxidant Properties of Underutilized Edible Flowers of Nelumbo nucifera and Nymphaea alba. International Journal of Health Sciences & Research (Www.ijhsr.org) International Journal of Health Sciences and Research, 2107(10), 210–223. Retrieved from http://www.ijhsr.org/IJHSR_Vol.7_Issue.10_Oct2017/30.pdf
  2. Adnaik, R. S., Pai, P. T., Sapakal, V. D., Naikwade, N. S., & Magdum, C. S. (2009). Anxiolytic activity of Vitex negundo Linn . in experimental models of anxiety in mice. (September), 243–247. https://doi.org/10.4103/0973-8258.56284
  3. Batool, R., Kalsoom, A., Akbar, I., Arshad, N., & Jamil, N. (2018). Antilisterial Effect of Rosa damascena and Nymphaea alba in Mus musculus. BioMed Research International, 2018. https://doi.org/10.1155/2018/4543723
  4. Bose, A., Ray, S., & Sahoo, M. (2012). Evaluation of analgesic and antioxidant potential of ethanolic extract of <i>Nymphaea alba</i> rhizome. Oxidants and Antioxidants in Medical Science, 1(3), 217. https://doi.org/10.5455/oams.140912.or.017
  5. Cudalbeanu, M., Ghinea, I. O., Furdui, B., Dah-Nouvlessounon, D., Raclea, R., Costache, T., … Dinica, R. M. (2018). Exploring new antioxidant and mineral compounds from nymphaea alba wild-grown in danube delta biosphere. Molecules, 23(6), 1–16. https://doi.org/10.3390/molecules23061247
  6. A, Mustaq., N,Iqbal., M,Jamil., NR,Khawaja., UF,Gohar dan MA,Mehmood. (2017). Antidiabetic and antihyperlidemic of aqueous ethanolic ekstract of mentha spicata (leaves), plumeria alba (leaves) and nymphaeae alba (flowers and rhizomes), International Journal of Biology, Pharmacy and Apllied Sciences, 6(1), 108–124.
  7. Madhusudhanan, N., Lakshmi, T., Gowtham, K. S., Ramakrishanan, N., Venu Gopala Rao, K., Roy, A., & Geetha, R. V. (2011). Invitro antioxidant and free radical scavenging activity of aqueous and ethanolic flower extract of Nymphaea alba. International Journal of Drug Development and Research, 3(3), 252–258.
  8. Nasiruddin, M., Khan, I. A., & Arif, S. H. (2018). Therapeutic effect of Nymphaea alba Linn. Flowers against isoniazid-inducedhepatotoxicity: An experimental study. Asian Journal of Pharmaceutical and Clinical Research, 11(5), 333–336. https://doi.org/10.22159/ajpcr.2018.v11i5.2283
  9. Ngwoke, K. G., Odimegwu, D. C., & Esimone, C. O. (2011). Antimicrobial natural products Antimicrobial resistance Epidemiology of resistance. Formatex, 1011–1026.
  10. Pareek, S. (2016). Chapter 17 – Nutritional and Biochemical Composition of Lychee (Litchi chinensis Sonn.) Cultivars. In Nutritional Composition of Fruit Cultivars (pp. 395–418). https://doi.org/10.1016/B978-0-12-408117-8.00017-9
  11. Riham, O. B., Reham, W., Noha, S., & Ibrahim, E. S. (2016). Characterization of the bioactive constituents of Nymphaea alba rhizomes and evaluation of anti-biofilm as well as antioxidant and cytotoxic properties. Journal of Medicinal Plants Research, 10(26), 390–401. https://doi.org/10.5897/jmpr2016.6162
  12. RS, J. J., Jagadeesh, S., Ganesan, S., K, V. R., & Eerike, M. (2013). Anti inflammatory activity of ethanolic extract of Nymphaea Alba flower in Swiss albino mice. International Journal of Medical Research & Health Sciences, 2(3), 474. https://doi.org/10.5958/j.2319-5886.2.3.082
  13. Saleem, M., Nazir, M., Ali, M. S., Hussain, H., Lee, Y. S., Riaz, N., & Jabbar, A. (2010). Antimicrobial natural products: An update on future antibiotic drug candidates. Natural Product Reports, 27(2), 238–254. https://doi.org/10.1039/b916096e
  14. Studies, R. (2017). UDS International Journal of Development [UDSIJD] Volume 4 No. 1, August, 2017 http://www.udsijd.org. 4(1), 46–63.
  15. WHO. Antimicrobial resistance. (2017). Retrieved from http://www.who.int/mediacentre/factsheets/fs194/en/#.UFDmbKuzN0w.mendeley
  16. Yildirim, A. B., Karakas, F. P., & Turker, A. U. (2013). In vitro antibacterial and antitumor activities of some medicinal plant extracts, growing in Turkey. Asian Pacific Journal of Tropical Medicine, 6(8), 616–624. https://doi.org/10.1016/S1995-7645(13)60106-6